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www.ahrq.gov/sites/default/files/wysiwyg/hai/tools/mrsa/167-hospital-level-gap-analysis.pdf
June 02, 2025 - Yes, cultures or tests positive for MRSA. … Yes, cultures or tests positive for MRSA. … Does your laboratory have the capacity to process surveillance cultures either on-site or by sending … Is active surveillance for MRSA performed (e.g., obtaining nasal swabs for culture at regular
intervals … : Off
Yes, cultures or tests positive for MRSA: Off
Yes, cultures or tests positive for other epidemiologically
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/best-practices/sepsis-slides.pptx
November 01, 2019 - 7
AHRQ Safety Program for Improving Antibiotic Use – Acute Care
Sepsis
Sepsis: Cultures
Blood cultures … critical component of sepsis management8
Daily assessment of patient status, source of infection, and culture … Three scenarios:
No evidence of infection and antibiotics can be stopped
Evidence of infection and culture … data are available to guide narrowing of therapy
Evidence of infection and no culture data are available … have grown, narrow based on culture results
22
AHRQ Safety Program for Improving Antibiotic Use
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/safety/changes-slides.pptx
November 01, 2019 - vancomycin and piperacillin/tazobactam
On day 2, he undergoes ERCP and the stone is removed
Blood cultures … Have I ordered appropriate cultures before starting antibiotics? … daily briefing about antibiotic regimen with team
Institutional factors are related to institution culture … revealed E. coli
Develop guidelines for intra-abdominal infections that discuss culture-directed therapy … and feedback for patients on vancomycin
Develop vancomycin auto-stops
Educate nurses about reviewing culture
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www.ahrq.gov/hai/cauti-tools/phys-championsgd/section3.html
October 01, 2015 - His blood cultures grew methicillin- resistant Staphylococcus aureus (MRSA). … Blood and urine cultures grew Escherichia coli . … Blood cultures grew Staphylococcus aureus , and the catheter tip grew the same organism. … The patient’s bacteremia persisted, and his repeat blood cultures continued to be positive for more than … His sputum culture grew Pseudomonas aeruginosa .
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www.ahrq.gov/sites/default/files/wysiwyg/hai/tools/mrsa/046-evidence-behind-decolonization-strategies-slides.pptx
October 01, 2024 - daily 2 percent CHG cloths
Results: 87 percent reduction in CLABSI and 41 percent decrease in blood culture … Additional Decolonization Impact
Additional benefits from subsequent analysis:
Reduction of blood culture … leave-on CHG bed bath
Twice-daily mupirocin for 5 days for patients who are MRSA-positive by history, culture … Does chlorhexidine bathing in adult intensive care units reduce blood culture contamination? … anatomical sites should be sampled for screening of methicillin-resistant Staphylococcus aureus carriage by culture
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/best-practices/HAP-one-page.docx
November 01, 2019 - should be considered when indicated
· Enterococcus species and Candida species that grow in sputum cultures … are highly likely to be colonizers and do not require treatment
· Obtain sputum Gram stain and culture … whenever possible
· Obtain blood cultures for severely ill patients
· Consider obtaining Legionella … pneumonia
· In most cases, stop antibiotics if viral respiratory testing is positive
· Use sputum culture … therapy was started and the beta-lactam is active against respiratory pathogen recovered
· If no sputum cultures
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/best-practices/VAP-slides.pptx
November 01, 2019 - Have I ordered appropriate cultures before starting antibiotics? … Have I ordered appropriate cultures before starting antibiotics? … Program for Improving Antibiotic Use – Acute Care
7
Moment 2: Diagnostic Tests for VAP
Noninvasive culture … recommended over invasive techniques like bronchoscopy1
Send respiratory specimen for Gram stain and culture … diagnosis is identified, stop VAP-targeted therapy
13
If VAP is the likely diagnosis, use respiratory culture
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www.ahrq.gov/sites/default/files/wysiwyg/hai/abate/introduction/decision-making.pdf
March 01, 2022 - clinical trials and adoption
after definitive trials must be determined based upon local needs and culture … Choose a 1-year
period and count the number of unique patients who have a positive blood culture
from … Use your current processes for identifying MRSA carriers using reported
history, prior culture result … The
order of approaching these key stakeholders will depend on the culture, standard
processes, and … cultures, and history of MRSA to target those with
devices
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www.ahrq.gov/antibiotic-use/long-term-care/best-practices/uti-assess.html
June 01, 2021 - Poster – Collecting Bacterial Cultures (PDF, 202.3 KB)
Poster – Urine Culture Collection (PDF, 178 … KB)
One-Page Document – Collection of Microbiological Cultures (PDF, 159.2 KB)
The materials
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/long-term-care/tracking-measuring-data-facilitator-guide.docx
June 01, 2021 - and reviews
a data collection form designed to facilitate tracking antibiotic use, orders for urine cultures … Facilities can use data on antibiotic use, orders for urine cultures, and C. difficile LabID events to … The form can also be used to keep track of C. difficile LabID events and the number of urine cultures … Another data measure to consider tracking is the number of urine cultures collected. … All urine cultures should be collected, even if they are not positive.
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/best-practices/HAP-facilitator-guide.pdf
November 01, 2019 - Always attempt to obtain a sputum Gram stain and
culture. … Blood cultures should be considered for severely ill
patients. … S. aureus and
gram-negative bacilli pathogens grow easily in culture. … If HAP is the likely diagnosis, use sputum culture results
to narrow therapy. … When cultures have not been obtained, directing
therapy can be challenging, and the decision to de-
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/long-term-care/four-moments-questions.pdf
June 01, 2021 - • Fever
• Productive cough
• Dysuria
• Purulence from skin
• Warm, red skin
Moment 2
Cultures & Empiric … Have we collected appropriate
cultures before starting
antibiotics?
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/long-term-care/four-moments-form.pdf
June 01, 2021 - DIRECTIONS: Use this form when a complete blood count (CBC), urinalysis/urine culture, or antibiotic … MOMENT 2
HAVE I ORDERED APPROPRIATE CULTURES … Were appropriate cultures ordered before antibiotics were started? … DIRECTIONS: Use this form when a complete blood count (CBC), urinalysis/urine culture, or antibiotic … MOMENT 2
HAVE I ORDERED APPROPRIATE CULTURES BEFORE STARTING ANTIBIOTICS?
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/long-term-care/poster-4x6-four-moments-double.pdf
June 01, 2021 - Have we
collected appropriate cultures before
starting antibiotics? … Have we
collected appropriate cultures before
starting antibiotics?
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www.ahrq.gov/sites/default/files/wysiwyg/professionals/quality-patient-safety/hais/cauti-tools/archived-webinars/mindfulness-061014.pptx
March 07, 2014 - and when and when not to treat positive urine cultures. … Combination
Efforts at CAUTI prevention often entail:
Improving urine culturing practices:
Only send cultures … Send the cultures the right way
Eliminating treatment of asymptomatic bacteruria. … Send U/A & urine culture
Document indication for sending urine culture
Start empiric therapy
(see … down to oral quinolone if susceptible
* Empiric choices should take into account recent previous cultures
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www.ahrq.gov/hai/cauti-tools/archived-webinars/mindfulness-slides.html
December 01, 2017 - and when and when not to treat positive urine cultures. … Efforts at CAUTI prevention often entail:
Improving urine culturing practices:
Only send cultures … Send the cultures the right way
Eliminating treatment of asymptomatic bacteruria. … Question
Does your hospital have guidelines that describe appropriate criteria for ordering urine cultures … * Empiric choices should take into account recent previous cultures*
Follow culture results and de-escalate
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www.ahrq.gov/sites/default/files/wysiwyg/hai/abate/huddle/iodophor-administration.docx
March 01, 2022 - methicillin-resistant Staphylococcus aureus (MRSA) carriers by history, screening tests, or clinical cultures … methicillin-resistant Staphylococcus aureus (MRSA) carriers by history, screening tests, or clinical cultures
-
www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/best-practices/diverticulitis-biliary-infections-facilitator-guide.pdf
November 01, 2019 - Have I ordered appropriate cultures before starting
antibiotics? … diagnosis in diverticulitis is
challenging unless the patient has an abscess that is
drained and sent for culture … Commentary Slide Number and Slide
Moment 3: Narrowing and Converting to
Oral Therapy
SAY:
If culture … Cultures of
bile during ERCP should also be obtained. … Remember to narrow therapy based on culture data, if
available.
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www.ahrq.gov/sites/default/files/wysiwyg/antibiotic-use/best-practices/HAP-slides.pptx
November 01, 2019 - Have I ordered appropriate cultures before starting antibiotics? … Have I ordered appropriate cultures before starting antibiotics? … enteric bacteria, Pseudomonas aeruginosa
Atypical organisms are uncommon
Obtain sputum Gram stain and culture … Have I ordered appropriate cultures before starting antibiotics? … Have I ordered appropriate cultures before starting antibiotics?
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www.ahrq.gov/research/findings/final-reports/stpra/stpraapa.html
April 01, 2018 - During operative process, culture collected. … Culture considered positive if > 30cfu of bacteria identified. … During the operative procedure, a semiquantitative culture was collected from subcutaneous fat in the … positive cultures had colony counts that were too numerous to count. … , compared with 82 of 1,576 patients (5.2%) with negative cultures.